Probably. Sometimes buyers come to me and say they have a certain score and later we find out that the score is not the same one used in mortgage lending.
Besides the scores (of which the middle one, not the highest one and not the lowest one, is used to qualify) certain items on the credit report can prevent you from getting a loan, such as a recent foreclosure, a recent short sale (neither of these may apply to you), a recent bankruptcy, multiple late-pays, and liens of many types.
In addition, your income must be provable and have a solid two year history or longer in the same profession. You can expect to qualify if you don't have problems like those mentioned, enough income so that the monthly payment plus all your other debt payments are less than about 2/5 of your gross income, and you have at least 5% in cash to put down.
Ask your loan officer to pre-qualify you and all will become apparent. Before you start looking it is better to know how much you qualify for. Since other debt payments, such as for student loans and credit cards, reduce the amount available for paying back the mortgage loan, you should expect your payment to fit into your overall budget.