You'll want to know what business model they follow. Some companies are traditional, others are 100% commission but you pay monthly desk fees and fees at closings.
How "hands on" is the broker in your real estate business? Some micro manage, others treat you as professionals who are independent contractors.
Ask to see and then read the office manual for agents. It spells out the expectations of the broker for his/her agents.
Ask to see the contract you will be expected to sign with the broker, read it carefully. Although you may not think it important now, pay careful attention to how your listings/customers are dealt with by the broker should you leave for another company.
You'll want to know what level of administrative support will be offered. Are you expected to input your own listings, procedure for processing listing and sale paperwork, etc.
Consider the location of the office or offices, number of agents, reputation of the company, branding of the company. Will working for that brokerage enhance your career or not?
Training. If you are inexperienced or new to FL real estate, I would definitely want to know what training and/or mentoring is available. Some franchise companies offer in house training.
These are some suggestions for your interview. I would suggest meeting with at least 3 companies before you make a decision.... more